m6a antibody Search Results


m6a  (Novus Biologicals)
93
Novus Biologicals m6a
M6a, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m6a/product/Novus Biologicals
Average 93 stars, based on 1 article reviews
m6a - by Bioz Stars, 2026-03
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anti m6a antibody  (MedChemExpress)
93
MedChemExpress anti m6a antibody
<t>M6A</t> analysis of HNF3γ mRNA in HCC. a The correlation between HNF3γ levels and METTL3, METTL14, WTAP, or FTO expression in HCC tissues was evaluated in 57 HCC specimens by Pearson’s correlation analysis. b The correlation between HNF3γ levels and METTL14 expression in HCC tissues from Cohort 1 was evaluated using a chi-square test. Representative IHC staining images are shown. Scale bar, 100 μm. c HCCLM3 and Huh7 cells were transfected with si-NC or siMETTL14 and then subjected to immunoprecipitation of m6A-modified RNA followed by real-time PCR analysis. d To analyze the effect of METTL14 on HNF3γ mRNA degradation, HCCLM3 or Huh7 cells transfected with si-NC or si-METTL14 were incubated with actinomycin D (10 μM) for 0, 15, 30, 60, 120, 180, 240, or 300 min. The levels of HNF3γ mRNA were determined by real-time PCR and the values were normalized to time zero. e Relative luciferase activity of pMIR-REPORT-HNF3γ-CDS (left panel) and pMIR-REPORT-HNF3γ-3′ UTR (right panel) with either wild-type or mutant (A-to-T mutation) m6A sites in HCCLM3 cells co-transfected with si-NC or si-METTL14, respectively. Firefly luciferase activity was measured and normalized to Renilla luciferase activity. Results were expressed as means ± SEM. * Indicates the significant difference between WT + si-NC group and WT + si-METTL14 group. # Indicates the significant difference between WT + si-NC group and MUT + si-NC group. See Supplementary materials for the details. f The expression of HNF3γ in HCCLM3 or Huh7 cells transfected with si-NC or si-IGF2BP1/2/3 was determined by real-time PCR. g RIP assay was carried out using anti-IGF2BP1/2/3 antibodies. The RNA was extracted from protein G-agarose with anti-IGF2BP1/2/3 antibody, or with control IgG. The specific anti-IGF2BP1/2/3 binding mRNA regions of HNF3γ were amplified by PCR
Anti M6a Antibody, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti m6a antibody/product/MedChemExpress
Average 93 stars, based on 1 article reviews
anti m6a antibody - by Bioz Stars, 2026-03
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oct4  (Proteintech)
96
Proteintech oct4
<t>M6A</t> analysis of HNF3γ mRNA in HCC. a The correlation between HNF3γ levels and METTL3, METTL14, WTAP, or FTO expression in HCC tissues was evaluated in 57 HCC specimens by Pearson’s correlation analysis. b The correlation between HNF3γ levels and METTL14 expression in HCC tissues from Cohort 1 was evaluated using a chi-square test. Representative IHC staining images are shown. Scale bar, 100 μm. c HCCLM3 and Huh7 cells were transfected with si-NC or siMETTL14 and then subjected to immunoprecipitation of m6A-modified RNA followed by real-time PCR analysis. d To analyze the effect of METTL14 on HNF3γ mRNA degradation, HCCLM3 or Huh7 cells transfected with si-NC or si-METTL14 were incubated with actinomycin D (10 μM) for 0, 15, 30, 60, 120, 180, 240, or 300 min. The levels of HNF3γ mRNA were determined by real-time PCR and the values were normalized to time zero. e Relative luciferase activity of pMIR-REPORT-HNF3γ-CDS (left panel) and pMIR-REPORT-HNF3γ-3′ UTR (right panel) with either wild-type or mutant (A-to-T mutation) m6A sites in HCCLM3 cells co-transfected with si-NC or si-METTL14, respectively. Firefly luciferase activity was measured and normalized to Renilla luciferase activity. Results were expressed as means ± SEM. * Indicates the significant difference between WT + si-NC group and WT + si-METTL14 group. # Indicates the significant difference between WT + si-NC group and MUT + si-NC group. See Supplementary materials for the details. f The expression of HNF3γ in HCCLM3 or Huh7 cells transfected with si-NC or si-IGF2BP1/2/3 was determined by real-time PCR. g RIP assay was carried out using anti-IGF2BP1/2/3 antibodies. The RNA was extracted from protein G-agarose with anti-IGF2BP1/2/3 antibody, or with control IgG. The specific anti-IGF2BP1/2/3 binding mRNA regions of HNF3γ were amplified by PCR
Oct4, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/oct4/product/Proteintech
Average 96 stars, based on 1 article reviews
oct4 - by Bioz Stars, 2026-03
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rabbit polyclonal anti gpm6a  (Proteintech)
93
Proteintech rabbit polyclonal anti gpm6a
<t>M6A</t> analysis of HNF3γ mRNA in HCC. a The correlation between HNF3γ levels and METTL3, METTL14, WTAP, or FTO expression in HCC tissues was evaluated in 57 HCC specimens by Pearson’s correlation analysis. b The correlation between HNF3γ levels and METTL14 expression in HCC tissues from Cohort 1 was evaluated using a chi-square test. Representative IHC staining images are shown. Scale bar, 100 μm. c HCCLM3 and Huh7 cells were transfected with si-NC or siMETTL14 and then subjected to immunoprecipitation of m6A-modified RNA followed by real-time PCR analysis. d To analyze the effect of METTL14 on HNF3γ mRNA degradation, HCCLM3 or Huh7 cells transfected with si-NC or si-METTL14 were incubated with actinomycin D (10 μM) for 0, 15, 30, 60, 120, 180, 240, or 300 min. The levels of HNF3γ mRNA were determined by real-time PCR and the values were normalized to time zero. e Relative luciferase activity of pMIR-REPORT-HNF3γ-CDS (left panel) and pMIR-REPORT-HNF3γ-3′ UTR (right panel) with either wild-type or mutant (A-to-T mutation) m6A sites in HCCLM3 cells co-transfected with si-NC or si-METTL14, respectively. Firefly luciferase activity was measured and normalized to Renilla luciferase activity. Results were expressed as means ± SEM. * Indicates the significant difference between WT + si-NC group and WT + si-METTL14 group. # Indicates the significant difference between WT + si-NC group and MUT + si-NC group. See Supplementary materials for the details. f The expression of HNF3γ in HCCLM3 or Huh7 cells transfected with si-NC or si-IGF2BP1/2/3 was determined by real-time PCR. g RIP assay was carried out using anti-IGF2BP1/2/3 antibodies. The RNA was extracted from protein G-agarose with anti-IGF2BP1/2/3 antibody, or with control IgG. The specific anti-IGF2BP1/2/3 binding mRNA regions of HNF3γ were amplified by PCR
Rabbit Polyclonal Anti Gpm6a, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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anti-m6a antibody 202003  (Synaptic Systems)
90
Synaptic Systems anti-m6a antibody 202003
<t>M6A</t> analysis of HNF3γ mRNA in HCC. a The correlation between HNF3γ levels and METTL3, METTL14, WTAP, or FTO expression in HCC tissues was evaluated in 57 HCC specimens by Pearson’s correlation analysis. b The correlation between HNF3γ levels and METTL14 expression in HCC tissues from Cohort 1 was evaluated using a chi-square test. Representative IHC staining images are shown. Scale bar, 100 μm. c HCCLM3 and Huh7 cells were transfected with si-NC or siMETTL14 and then subjected to immunoprecipitation of m6A-modified RNA followed by real-time PCR analysis. d To analyze the effect of METTL14 on HNF3γ mRNA degradation, HCCLM3 or Huh7 cells transfected with si-NC or si-METTL14 were incubated with actinomycin D (10 μM) for 0, 15, 30, 60, 120, 180, 240, or 300 min. The levels of HNF3γ mRNA were determined by real-time PCR and the values were normalized to time zero. e Relative luciferase activity of pMIR-REPORT-HNF3γ-CDS (left panel) and pMIR-REPORT-HNF3γ-3′ UTR (right panel) with either wild-type or mutant (A-to-T mutation) m6A sites in HCCLM3 cells co-transfected with si-NC or si-METTL14, respectively. Firefly luciferase activity was measured and normalized to Renilla luciferase activity. Results were expressed as means ± SEM. * Indicates the significant difference between WT + si-NC group and WT + si-METTL14 group. # Indicates the significant difference between WT + si-NC group and MUT + si-NC group. See Supplementary materials for the details. f The expression of HNF3γ in HCCLM3 or Huh7 cells transfected with si-NC or si-IGF2BP1/2/3 was determined by real-time PCR. g RIP assay was carried out using anti-IGF2BP1/2/3 antibodies. The RNA was extracted from protein G-agarose with anti-IGF2BP1/2/3 antibody, or with control IgG. The specific anti-IGF2BP1/2/3 binding mRNA regions of HNF3γ were amplified by PCR
Anti M6a Antibody 202003, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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m6a antibody abe572  (Merck KGaA)
90
Merck KGaA m6a antibody abe572
<t>M6A</t> analysis of HNF3γ mRNA in HCC. a The correlation between HNF3γ levels and METTL3, METTL14, WTAP, or FTO expression in HCC tissues was evaluated in 57 HCC specimens by Pearson’s correlation analysis. b The correlation between HNF3γ levels and METTL14 expression in HCC tissues from Cohort 1 was evaluated using a chi-square test. Representative IHC staining images are shown. Scale bar, 100 μm. c HCCLM3 and Huh7 cells were transfected with si-NC or siMETTL14 and then subjected to immunoprecipitation of m6A-modified RNA followed by real-time PCR analysis. d To analyze the effect of METTL14 on HNF3γ mRNA degradation, HCCLM3 or Huh7 cells transfected with si-NC or si-METTL14 were incubated with actinomycin D (10 μM) for 0, 15, 30, 60, 120, 180, 240, or 300 min. The levels of HNF3γ mRNA were determined by real-time PCR and the values were normalized to time zero. e Relative luciferase activity of pMIR-REPORT-HNF3γ-CDS (left panel) and pMIR-REPORT-HNF3γ-3′ UTR (right panel) with either wild-type or mutant (A-to-T mutation) m6A sites in HCCLM3 cells co-transfected with si-NC or si-METTL14, respectively. Firefly luciferase activity was measured and normalized to Renilla luciferase activity. Results were expressed as means ± SEM. * Indicates the significant difference between WT + si-NC group and WT + si-METTL14 group. # Indicates the significant difference between WT + si-NC group and MUT + si-NC group. See Supplementary materials for the details. f The expression of HNF3γ in HCCLM3 or Huh7 cells transfected with si-NC or si-IGF2BP1/2/3 was determined by real-time PCR. g RIP assay was carried out using anti-IGF2BP1/2/3 antibodies. The RNA was extracted from protein G-agarose with anti-IGF2BP1/2/3 antibody, or with control IgG. The specific anti-IGF2BP1/2/3 binding mRNA regions of HNF3γ were amplified by PCR
M6a Antibody Abe572, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibody specific to m6a 202003  (Synaptic Systems)
90
Synaptic Systems antibody specific to m6a 202003
<t>M6A</t> analysis of HNF3γ mRNA in HCC. a The correlation between HNF3γ levels and METTL3, METTL14, WTAP, or FTO expression in HCC tissues was evaluated in 57 HCC specimens by Pearson’s correlation analysis. b The correlation between HNF3γ levels and METTL14 expression in HCC tissues from Cohort 1 was evaluated using a chi-square test. Representative IHC staining images are shown. Scale bar, 100 μm. c HCCLM3 and Huh7 cells were transfected with si-NC or siMETTL14 and then subjected to immunoprecipitation of m6A-modified RNA followed by real-time PCR analysis. d To analyze the effect of METTL14 on HNF3γ mRNA degradation, HCCLM3 or Huh7 cells transfected with si-NC or si-METTL14 were incubated with actinomycin D (10 μM) for 0, 15, 30, 60, 120, 180, 240, or 300 min. The levels of HNF3γ mRNA were determined by real-time PCR and the values were normalized to time zero. e Relative luciferase activity of pMIR-REPORT-HNF3γ-CDS (left panel) and pMIR-REPORT-HNF3γ-3′ UTR (right panel) with either wild-type or mutant (A-to-T mutation) m6A sites in HCCLM3 cells co-transfected with si-NC or si-METTL14, respectively. Firefly luciferase activity was measured and normalized to Renilla luciferase activity. Results were expressed as means ± SEM. * Indicates the significant difference between WT + si-NC group and WT + si-METTL14 group. # Indicates the significant difference between WT + si-NC group and MUT + si-NC group. See Supplementary materials for the details. f The expression of HNF3γ in HCCLM3 or Huh7 cells transfected with si-NC or si-IGF2BP1/2/3 was determined by real-time PCR. g RIP assay was carried out using anti-IGF2BP1/2/3 antibodies. The RNA was extracted from protein G-agarose with anti-IGF2BP1/2/3 antibody, or with control IgG. The specific anti-IGF2BP1/2/3 binding mRNA regions of HNF3γ were amplified by PCR
Antibody Specific To M6a 202003, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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m6a antibody  (ABclonal Biotechnology)
90
ABclonal Biotechnology m6a antibody
<t>M6A</t> analysis of HNF3γ mRNA in HCC. a The correlation between HNF3γ levels and METTL3, METTL14, WTAP, or FTO expression in HCC tissues was evaluated in 57 HCC specimens by Pearson’s correlation analysis. b The correlation between HNF3γ levels and METTL14 expression in HCC tissues from Cohort 1 was evaluated using a chi-square test. Representative IHC staining images are shown. Scale bar, 100 μm. c HCCLM3 and Huh7 cells were transfected with si-NC or siMETTL14 and then subjected to immunoprecipitation of m6A-modified RNA followed by real-time PCR analysis. d To analyze the effect of METTL14 on HNF3γ mRNA degradation, HCCLM3 or Huh7 cells transfected with si-NC or si-METTL14 were incubated with actinomycin D (10 μM) for 0, 15, 30, 60, 120, 180, 240, or 300 min. The levels of HNF3γ mRNA were determined by real-time PCR and the values were normalized to time zero. e Relative luciferase activity of pMIR-REPORT-HNF3γ-CDS (left panel) and pMIR-REPORT-HNF3γ-3′ UTR (right panel) with either wild-type or mutant (A-to-T mutation) m6A sites in HCCLM3 cells co-transfected with si-NC or si-METTL14, respectively. Firefly luciferase activity was measured and normalized to Renilla luciferase activity. Results were expressed as means ± SEM. * Indicates the significant difference between WT + si-NC group and WT + si-METTL14 group. # Indicates the significant difference between WT + si-NC group and MUT + si-NC group. See Supplementary materials for the details. f The expression of HNF3γ in HCCLM3 or Huh7 cells transfected with si-NC or si-IGF2BP1/2/3 was determined by real-time PCR. g RIP assay was carried out using anti-IGF2BP1/2/3 antibodies. The RNA was extracted from protein G-agarose with anti-IGF2BP1/2/3 antibody, or with control IgG. The specific anti-IGF2BP1/2/3 binding mRNA regions of HNF3γ were amplified by PCR
M6a Antibody, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-m 6 a antibody  (Synaptic Systems)
90
Synaptic Systems anti-m 6 a antibody
<t>M6A</t> analysis of HNF3γ mRNA in HCC. a The correlation between HNF3γ levels and METTL3, METTL14, WTAP, or FTO expression in HCC tissues was evaluated in 57 HCC specimens by Pearson’s correlation analysis. b The correlation between HNF3γ levels and METTL14 expression in HCC tissues from Cohort 1 was evaluated using a chi-square test. Representative IHC staining images are shown. Scale bar, 100 μm. c HCCLM3 and Huh7 cells were transfected with si-NC or siMETTL14 and then subjected to immunoprecipitation of m6A-modified RNA followed by real-time PCR analysis. d To analyze the effect of METTL14 on HNF3γ mRNA degradation, HCCLM3 or Huh7 cells transfected with si-NC or si-METTL14 were incubated with actinomycin D (10 μM) for 0, 15, 30, 60, 120, 180, 240, or 300 min. The levels of HNF3γ mRNA were determined by real-time PCR and the values were normalized to time zero. e Relative luciferase activity of pMIR-REPORT-HNF3γ-CDS (left panel) and pMIR-REPORT-HNF3γ-3′ UTR (right panel) with either wild-type or mutant (A-to-T mutation) m6A sites in HCCLM3 cells co-transfected with si-NC or si-METTL14, respectively. Firefly luciferase activity was measured and normalized to Renilla luciferase activity. Results were expressed as means ± SEM. * Indicates the significant difference between WT + si-NC group and WT + si-METTL14 group. # Indicates the significant difference between WT + si-NC group and MUT + si-NC group. See Supplementary materials for the details. f The expression of HNF3γ in HCCLM3 or Huh7 cells transfected with si-NC or si-IGF2BP1/2/3 was determined by real-time PCR. g RIP assay was carried out using anti-IGF2BP1/2/3 antibodies. The RNA was extracted from protein G-agarose with anti-IGF2BP1/2/3 antibody, or with control IgG. The specific anti-IGF2BP1/2/3 binding mRNA regions of HNF3γ were amplified by PCR
Anti M 6 A Antibody, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-n 6-methyladenosine  (Synaptic Systems)
90
Synaptic Systems anti-n 6-methyladenosine
<t>M6A</t> analysis of HNF3γ mRNA in HCC. a The correlation between HNF3γ levels and METTL3, METTL14, WTAP, or FTO expression in HCC tissues was evaluated in 57 HCC specimens by Pearson’s correlation analysis. b The correlation between HNF3γ levels and METTL14 expression in HCC tissues from Cohort 1 was evaluated using a chi-square test. Representative IHC staining images are shown. Scale bar, 100 μm. c HCCLM3 and Huh7 cells were transfected with si-NC or siMETTL14 and then subjected to immunoprecipitation of m6A-modified RNA followed by real-time PCR analysis. d To analyze the effect of METTL14 on HNF3γ mRNA degradation, HCCLM3 or Huh7 cells transfected with si-NC or si-METTL14 were incubated with actinomycin D (10 μM) for 0, 15, 30, 60, 120, 180, 240, or 300 min. The levels of HNF3γ mRNA were determined by real-time PCR and the values were normalized to time zero. e Relative luciferase activity of pMIR-REPORT-HNF3γ-CDS (left panel) and pMIR-REPORT-HNF3γ-3′ UTR (right panel) with either wild-type or mutant (A-to-T mutation) m6A sites in HCCLM3 cells co-transfected with si-NC or si-METTL14, respectively. Firefly luciferase activity was measured and normalized to Renilla luciferase activity. Results were expressed as means ± SEM. * Indicates the significant difference between WT + si-NC group and WT + si-METTL14 group. # Indicates the significant difference between WT + si-NC group and MUT + si-NC group. See Supplementary materials for the details. f The expression of HNF3γ in HCCLM3 or Huh7 cells transfected with si-NC or si-IGF2BP1/2/3 was determined by real-time PCR. g RIP assay was carried out using anti-IGF2BP1/2/3 antibodies. The RNA was extracted from protein G-agarose with anti-IGF2BP1/2/3 antibody, or with control IgG. The specific anti-IGF2BP1/2/3 binding mRNA regions of HNF3γ were amplified by PCR
Anti N 6 Methyladenosine, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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m6a antibody  (Huabio Inc)
90
Huabio Inc m6a antibody
<t>M6A</t> analysis of HNF3γ mRNA in HCC. a The correlation between HNF3γ levels and METTL3, METTL14, WTAP, or FTO expression in HCC tissues was evaluated in 57 HCC specimens by Pearson’s correlation analysis. b The correlation between HNF3γ levels and METTL14 expression in HCC tissues from Cohort 1 was evaluated using a chi-square test. Representative IHC staining images are shown. Scale bar, 100 μm. c HCCLM3 and Huh7 cells were transfected with si-NC or siMETTL14 and then subjected to immunoprecipitation of m6A-modified RNA followed by real-time PCR analysis. d To analyze the effect of METTL14 on HNF3γ mRNA degradation, HCCLM3 or Huh7 cells transfected with si-NC or si-METTL14 were incubated with actinomycin D (10 μM) for 0, 15, 30, 60, 120, 180, 240, or 300 min. The levels of HNF3γ mRNA were determined by real-time PCR and the values were normalized to time zero. e Relative luciferase activity of pMIR-REPORT-HNF3γ-CDS (left panel) and pMIR-REPORT-HNF3γ-3′ UTR (right panel) with either wild-type or mutant (A-to-T mutation) m6A sites in HCCLM3 cells co-transfected with si-NC or si-METTL14, respectively. Firefly luciferase activity was measured and normalized to Renilla luciferase activity. Results were expressed as means ± SEM. * Indicates the significant difference between WT + si-NC group and WT + si-METTL14 group. # Indicates the significant difference between WT + si-NC group and MUT + si-NC group. See Supplementary materials for the details. f The expression of HNF3γ in HCCLM3 or Huh7 cells transfected with si-NC or si-IGF2BP1/2/3 was determined by real-time PCR. g RIP assay was carried out using anti-IGF2BP1/2/3 antibodies. The RNA was extracted from protein G-agarose with anti-IGF2BP1/2/3 antibody, or with control IgG. The specific anti-IGF2BP1/2/3 binding mRNA regions of HNF3γ were amplified by PCR
M6a Antibody, supplied by Huabio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibody against m6a rna #202 111  (Synaptic Systems)
90
Synaptic Systems antibody against m6a rna #202 111
<t>M6A</t> analysis of HNF3γ mRNA in HCC. a The correlation between HNF3γ levels and METTL3, METTL14, WTAP, or FTO expression in HCC tissues was evaluated in 57 HCC specimens by Pearson’s correlation analysis. b The correlation between HNF3γ levels and METTL14 expression in HCC tissues from Cohort 1 was evaluated using a chi-square test. Representative IHC staining images are shown. Scale bar, 100 μm. c HCCLM3 and Huh7 cells were transfected with si-NC or siMETTL14 and then subjected to immunoprecipitation of m6A-modified RNA followed by real-time PCR analysis. d To analyze the effect of METTL14 on HNF3γ mRNA degradation, HCCLM3 or Huh7 cells transfected with si-NC or si-METTL14 were incubated with actinomycin D (10 μM) for 0, 15, 30, 60, 120, 180, 240, or 300 min. The levels of HNF3γ mRNA were determined by real-time PCR and the values were normalized to time zero. e Relative luciferase activity of pMIR-REPORT-HNF3γ-CDS (left panel) and pMIR-REPORT-HNF3γ-3′ UTR (right panel) with either wild-type or mutant (A-to-T mutation) m6A sites in HCCLM3 cells co-transfected with si-NC or si-METTL14, respectively. Firefly luciferase activity was measured and normalized to Renilla luciferase activity. Results were expressed as means ± SEM. * Indicates the significant difference between WT + si-NC group and WT + si-METTL14 group. # Indicates the significant difference between WT + si-NC group and MUT + si-NC group. See Supplementary materials for the details. f The expression of HNF3γ in HCCLM3 or Huh7 cells transfected with si-NC or si-IGF2BP1/2/3 was determined by real-time PCR. g RIP assay was carried out using anti-IGF2BP1/2/3 antibodies. The RNA was extracted from protein G-agarose with anti-IGF2BP1/2/3 antibody, or with control IgG. The specific anti-IGF2BP1/2/3 binding mRNA regions of HNF3γ were amplified by PCR
Antibody Against M6a Rna #202 111, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


M6A analysis of HNF3γ mRNA in HCC. a The correlation between HNF3γ levels and METTL3, METTL14, WTAP, or FTO expression in HCC tissues was evaluated in 57 HCC specimens by Pearson’s correlation analysis. b The correlation between HNF3γ levels and METTL14 expression in HCC tissues from Cohort 1 was evaluated using a chi-square test. Representative IHC staining images are shown. Scale bar, 100 μm. c HCCLM3 and Huh7 cells were transfected with si-NC or siMETTL14 and then subjected to immunoprecipitation of m6A-modified RNA followed by real-time PCR analysis. d To analyze the effect of METTL14 on HNF3γ mRNA degradation, HCCLM3 or Huh7 cells transfected with si-NC or si-METTL14 were incubated with actinomycin D (10 μM) for 0, 15, 30, 60, 120, 180, 240, or 300 min. The levels of HNF3γ mRNA were determined by real-time PCR and the values were normalized to time zero. e Relative luciferase activity of pMIR-REPORT-HNF3γ-CDS (left panel) and pMIR-REPORT-HNF3γ-3′ UTR (right panel) with either wild-type or mutant (A-to-T mutation) m6A sites in HCCLM3 cells co-transfected with si-NC or si-METTL14, respectively. Firefly luciferase activity was measured and normalized to Renilla luciferase activity. Results were expressed as means ± SEM. * Indicates the significant difference between WT + si-NC group and WT + si-METTL14 group. # Indicates the significant difference between WT + si-NC group and MUT + si-NC group. See Supplementary materials for the details. f The expression of HNF3γ in HCCLM3 or Huh7 cells transfected with si-NC or si-IGF2BP1/2/3 was determined by real-time PCR. g RIP assay was carried out using anti-IGF2BP1/2/3 antibodies. The RNA was extracted from protein G-agarose with anti-IGF2BP1/2/3 antibody, or with control IgG. The specific anti-IGF2BP1/2/3 binding mRNA regions of HNF3γ were amplified by PCR

Journal: Signal Transduction and Targeted Therapy

Article Title: m6A RNA methylation-mediated HNF3γ reduction renders hepatocellular carcinoma dedifferentiation and sorafenib resistance

doi: 10.1038/s41392-020-00299-0

Figure Lengend Snippet: M6A analysis of HNF3γ mRNA in HCC. a The correlation between HNF3γ levels and METTL3, METTL14, WTAP, or FTO expression in HCC tissues was evaluated in 57 HCC specimens by Pearson’s correlation analysis. b The correlation between HNF3γ levels and METTL14 expression in HCC tissues from Cohort 1 was evaluated using a chi-square test. Representative IHC staining images are shown. Scale bar, 100 μm. c HCCLM3 and Huh7 cells were transfected with si-NC or siMETTL14 and then subjected to immunoprecipitation of m6A-modified RNA followed by real-time PCR analysis. d To analyze the effect of METTL14 on HNF3γ mRNA degradation, HCCLM3 or Huh7 cells transfected with si-NC or si-METTL14 were incubated with actinomycin D (10 μM) for 0, 15, 30, 60, 120, 180, 240, or 300 min. The levels of HNF3γ mRNA were determined by real-time PCR and the values were normalized to time zero. e Relative luciferase activity of pMIR-REPORT-HNF3γ-CDS (left panel) and pMIR-REPORT-HNF3γ-3′ UTR (right panel) with either wild-type or mutant (A-to-T mutation) m6A sites in HCCLM3 cells co-transfected with si-NC or si-METTL14, respectively. Firefly luciferase activity was measured and normalized to Renilla luciferase activity. Results were expressed as means ± SEM. * Indicates the significant difference between WT + si-NC group and WT + si-METTL14 group. # Indicates the significant difference between WT + si-NC group and MUT + si-NC group. See Supplementary materials for the details. f The expression of HNF3γ in HCCLM3 or Huh7 cells transfected with si-NC or si-IGF2BP1/2/3 was determined by real-time PCR. g RIP assay was carried out using anti-IGF2BP1/2/3 antibodies. The RNA was extracted from protein G-agarose with anti-IGF2BP1/2/3 antibody, or with control IgG. The specific anti-IGF2BP1/2/3 binding mRNA regions of HNF3γ were amplified by PCR

Article Snippet: Magnetic beads (MedChemExpress) were incubated with anti-m6A antibody for 30 min at room temperature.

Techniques: Expressing, Immunohistochemistry, Transfection, Immunoprecipitation, Modification, Real-time Polymerase Chain Reaction, Incubation, Luciferase, Activity Assay, Mutagenesis, Control, Binding Assay, Amplification